I feel that our challenges within the experiment are going to include keeping our data based solely off of just the chemicals. We need to be extremely cautious about the way that we treat them with all other features before and during the project. Some things that we are going to need to monitor so closely that are going to serve as a challenge would include, regulating the temperature within the box, assuring that the light they are under is switched on/off at an exact 12 hour schedule as well as doing our best to keep them as undisturbed as possible and giving each of them an equal amount of interaction.
One thing that concerns me is the possibility of the experiment turning in the direction of the dinoflagelettes dying at the first initiation of the chemicals that we put into the test tubes. If this is the case we would know that they can only survive in pure salt water. Although we do know that they will not be able to survive in this form of water to a certain extent I would like to be able to test how MUCH chemicals they can handle to survive.
This is a photo of our bioluminescent dinoflagelettes glowing before they have been exposed to any form of chemicals.